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Alpha-1-antitrypsin acts as a preeclampsia-related protein: A proteomic study

Gynecologic and Obstetric Investigation, ISSN: 0378-7346, Vol: 73, Issue: 3, Page: 252-259
2012
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Article Description

Aims: To screen the preeclampsia-related protein by proteomics. Methods: Proteomics was performed to identify differential protein expression profiles between normal full-term pregnancy, early-onset severe preeclampsia (ES-PE) or late-onset severe preeclampsia (LS-PE; n = 10 per group). Real-time quantitative PCR and immunohistochemistry were conducted to confirm the expression of α -antitrypsin (α -AT) in the decidual tissues of different subjects. ELISA was employed to detect the α -AT content in the peripheral blood of 90 women (n = 30 per group). Results: We successfully constructed two-dimensional electrophoresis maps of decidual tissues, and a total of 20 differentially expressed proteins were identified. The α -AT expression was different among the three groups. The normal full-term pregnancy women expressed the most α -AT, and the LS-PE women expressed the least amount of α -AT. The difference in the α -AT expression was consistent with the proteomics data. The peripheral α -AT content was the highest in the normal full-term pregnancy group (1.85 ± 0.15 g/l), moderate in the ES-PE group (0.77 ± 0.14 g/l) and lowest in the LS-PE group (0.42 ± 0.07 g/l; p < 0.05). Conclusion: Using 2D PAGE, we identified twenty proteins with significantly altered expression in PE. These differentially expressed proteins include prevention protein, in which α -AT is downregulated in PE. © 2012 S. Karger AG, Basel.

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