PlumX Metrics
Embed PlumX Metrics

Synthesis and characterization of polymer-(multi)-peptide conjugates for control of specific cell aggregation

Journal of Biomaterials Science, Polymer Edition, ISSN: 1568-5624, Vol: 9, Issue: 3, Page: 207-226
1998
  • 32
    Citations
  • 0
    Usage
  • 10
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

A new synthetic approach has been applied to obtain novel di-, tetra-, and (multi)-peptide containing polymer conjugates in quantitative yields with a high degree of conjugation. Bis-(N-hydroxysuccinimidyl) esters of PEG (M= 200, 600, 1400, 2000, and 3400) were synthesized and studied in a condensation reaction with synthetic peptides: glycine-glycine-tyrosine-arginine (GGYR), a model peptide, and glycine-arginine-glycine-aspartic acid-tyrosine (GRGDY), a sequence known to promote cell adhesion and aggregation. Tetra-substituted derivatives of PEG-based conjugates were synthesized by coupling L-aspartic acid and L-aspartyl-L-phenylalanine through a condensation procedure in organic media. Poly(acrylic acid) and co-polymers (M= 2000 and 5000) were studied as a model of multifunctional linear polymers in the reaction with L-tryptophan and GGYR. Alternative polymer-(multi)-peptide conjugates were successfully synthesized using Starburst® dendrimer PAMAM (G = 3), ’short’ and ’long’-chain PEG-based active esters and GRGDY. The structure of the intermediate precursors and peptide-conjugates was confirmed by spectral (UV-Vis, FTIRH-NMR) and chromatographic (RP-HPLC and SEC) methods. By varying the properties of the interconnecting polymer - such as hydrophobicity, molecular weight, and functionality - a set of polymer-GRGDY conjugates was synthesized. © VSP 1998.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know