Molecular profiles of Quadriceps muscle in myostatin-null mice reveal PI3K and apoptotic pathways as myostatin targets
BMC Genomics, ISSN: 1471-2164, Vol: 10, Issue: 1, Page: 196
2009
- 72Citations
- 88Usage
- 54Captures
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Metrics Details
- Citations72
- Citation Indexes72
- 72
- CrossRef54
- Usage88
- Downloads87
- Abstract Views1
- Captures54
- Readers54
- 54
Article Description
Background: Myostatin (MSTN), a member of the TGF-β superfamily, has been identified as a negative regulator of skeletal muscle mass. Inactivating mutations in the MSTN gene are responsible for the development of a hypermuscular phenotype. In this study, we performed transcriptomic and proteomic analyses to detect altered expression/ abundance of genes and proteins. These differentially expressed genes and proteins may represent new molecular targets of MSTN and could be involved in the regulation of skeletal muscle mass. Results: Transcriptomic analysis of the Quadriceps muscles of 5-week-old MSTN-null mice (n = 4) and their controls (n = 4) was carried out using microarray (human and murine oligonucleotide sequences) of 6,473 genes expressed in muscle. Proteomic profiles were analysed using two-dimensional gel electrophoresis coupled with mass spectrometry. Comparison of the transcriptomic profiles revealed 192 up- and 245 down- regulated genes. Genes involved in the PI3K pathway, insulin/IGF pathway, carbohydrate metabolism and apoptosis regulation were up-regulated. Genes belonging to canonical Wnt, calcium signalling pathways and cytokine-receptor cytokine interaction were down-regulated. Comparison of the protein profiles revealed 20 up- and 18 down-regulated proteins spots. Knockout of the MSTN gene was associated with up-regulation of proteins involved in glycolytic shift of the muscles and down-regulation of proteins involved in oxidative energy metabolism. In addition, an increased abundance of survival/anti-apoptotic factors were observed. Conclusion: All together, these results showed a differential expression of genes and proteins related to the muscle energy metabolism and cell survival/anti-apoptotic pathway (e.g. DJ-1, PINK1, 14-3-3ε protein, TCTP/GSK-3β). They revealed the PI3K and apoptotic pathways as MSTN targets and are in favour of a role of MSTN as a modulator of cell survival in vivo. © 2009 Chelh et al; licensee BioMed Central Ltd.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=66049084853&origin=inward; http://dx.doi.org/10.1186/1471-2164-10-196; http://www.ncbi.nlm.nih.gov/pubmed/19397818; https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-10-196; https://lib.dr.iastate.edu/ans_pubs/130; https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1130&context=ans_pubs; https://dx.doi.org/10.1186/1471-2164-10-196
Springer Science and Business Media LLC
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