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The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination

BMC Genomics, ISSN: 1471-2164, Vol: 8, Issue: 1, Page: 100
2007
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Article Description

Background: Conidia are considered to be the primary cause of infections by Trichophyton rubrum. Results: We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altered in the process were obtained and hierarchically clustered with respect to their expression profiles. By functional analysis, we provided a global view of an important biological system related to conidial germination, including characterization of the pattern of gene expression at sequential developmental phases, and changes of gene expression profiles corresponding to morphological transitions. We matched the EST sequences to GO terms in the Saccharomyces Genome Database (SGD). A number of homologues of Saccharomyces cerevisiae genes related to signalling pathways and some important cellular processes were found to be involved in T. rubrum germination. These genes and signalling pathways may play roles in distinct steps, such as activating conidial germination, maintenance of isotropic growth, establishment of cell polarity and morphological transitions. Conclusion: Our results may provide insights into molecular mechanisms of conidial germination at the cell level, and may enhance our understanding of regulation of gene expression related to the morphological construction of T. rubrum. © 2007 Liu et al; licensee BioMed Central Ltd.

Bibliographic Details

Liu, Tao; Zhang, Qian; Wang, Lingling; Yu, Lu; Leng, Wenchuan; Yang, Jian; Chen, Lihong; Peng, Junping; Ma, Li; Dong, Jie; Xu, Xingye; Xue, Ying; Zhu, Yafang; Zhang, Wenliang; Yang, Li; Li, Weijun; Sun, Lilian; Wan, Zhe; Ding, Guohui; Yu, Fudong; Tu, Kang; Qian, Ziliang; Li, Ruoyu; Shen, Yan; Li, Yixue; Jin, Qi

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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