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Towards rapid genotyping of resistant malaria parasites: Could loop-mediated isothermal amplification be the solution?

Malaria Journal, ISSN: 1475-2875, Vol: 13, Issue: 1, Page: 237
2014
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Review Description

Loop-mediated isothermal amplification (LAMP) is an innovative molecular technique that has been validated for point-of-care testing to diagnose malaria. Molecular detection and tracking of anti-malarial drug resistance is mainly based on highly sophisticated, costly and time-consuming techniques. With the validation of resistance-associated gene mutations in malaria parasites, there is a need to develop rapid, easy-to-use molecular tests for anti-malarial drug resistance genotyping. LAMP could be further developed as a point-of-care test to rapidly detect anti-malarial drug resistance-associated molecular markers, thereby help detecting and monitoring drug resistance in surveillance studies. © 2014 Abdul-Ghani; licensee BioMed Central Ltd.

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