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Generation of medaka gene knockout models by target-selected mutagenesis

Genome Biology, ISSN: 1474-7596, Vol: 7, Issue: 12, Page: R116
2006
  • 132
    Citations
  • 0
    Usage
  • 92
    Captures
  • 1
    Mentions
  • 9
    Social Media
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Metrics Details

  • Citations
    132
  • Captures
    92
  • Mentions
    1
    • References
      1
      • Wikipedia
        1
  • Social Media
    9
    • Shares, Likes & Comments
      9
      • Facebook
        9

Article Description

We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes. © 2006 Taniguchi et al.; licensee BioMed Central Ltd.

Bibliographic Details

Taniguchi, Yoshihito; Takeda, Shunichi; Furutani-Seiki, Makoto; Kamei, Yasuhiro; Todo, Takeshi; Sasado, Takao; Deguchi, Tomonori; Kondoh, Hisato; Mudde, Josine; Yamazoe, Mitsuyoshi; Hidaka, Masayuki; Mitani, Hiroshi; Toyoda, Atsushi; Sakaki, Yoshiyuki; Plasterk, Ronald H A; Cuppen, Edwin

Springer Science and Business Media LLC

Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology

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