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Searching for SNPs with cloud computing

Genome Biology, ISSN: 1474-7596, Vol: 10, Issue: 11, Page: R134
2009
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Most Recent News

Next-generation sequencing of Coccidioides immitis isolated during cluster investigation

Genotyping of microorganisms typically relies on comparison of genomic features (e.g., fragment size, repeats, single-nucleotide polymorphisms [SNPs]) between strains and/or against a database of feature

Article Description

As DNA sequencing outpaces improvements in computer speed, there is a critical need to accelerate tasks like alignment and SNP calling. Crossbow is a cloud-computing software tool that combines the aligner Bowtie and the SNP caller SOAPsnp. Executing in parallel using Hadoop, Crossbow analyzes data comprising 38-fold coverage of the human genome in three hours using a 320-CPU cluster rented from a cloud computing service for about $85. Crossbow is available from http://bowtie-bio.sourceforge.net/crossbow/.© 2009 Langmead et al; licensee BioMed Central Ltd.

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