Thionin-like peptide from Capsicum annuum fruits: Mechanism of action and synergism with fluconazole against Candida species Applied microbiology
BMC Microbiology, ISSN: 1471-2180, Vol: 16, Issue: 1, Page: 12
2016
- 70Citations
- 76Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations70
- Citation Indexes70
- 70
- CrossRef31
- Captures76
- Readers76
- 76
Article Description
Background: Thionins are a family of plant antimicrobial peptides (AMPs), which participate in plant defense system against pathogens. Here we describe some aspects of the CaThi thionin-like action mechanism, previously isolated from Capsicum annuum fruits. Thionin-like peptide was submitted to antimicrobial activity assays against Candida species for IC determination and synergism with fluconazole evaluation. Viability and plasma membrane permeabilization assays, induction of intracellular ROS production analysis and CaThi localization in yeast cells were also investigated. Results: CaThi had strong antimicrobial activity against six tested pathogenic Candida species, with IC ranging from 10 to 40 μg.mL. CaThi antimicrobial activity on Candida species was candidacidal. Moreover, CaThi caused plasma membrane permeabilization in all yeasts tested and induces oxidative stresses only in Candida tropicalis. CaThi was intracellularly localized in C. albicans and C. tropicalis, however localized in nuclei in C. tropicalis, suggesting a possible nuclear target. CaThi performed synergistically with fluconazole inhibiting all tested yeasts, reaching 100 % inhibition in C. parapsilosis. The inhibiting concentrations for the synergic pair ranged from 1.3 to 4.0 times below CaThi IC and from zero to 2.0 times below fluconazole IC Conclusion: The results reported herein may ultimately contribute to future efforts aiming to employ this plant-derived AMP as a new therapeutic substance against yeasts.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84956690211&origin=inward; http://dx.doi.org/10.1186/s12866-016-0626-6; http://www.ncbi.nlm.nih.gov/pubmed/26819228; http://www.biomedcentral.com/1471-2180/16/12; https://dx.doi.org/10.1186/s12866-016-0626-6; https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0626-6
Springer Science and Business Media LLC
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