PlumX Metrics
Embed PlumX Metrics

GAF domain is essential for nitrate-dependent AtNLP7 function

BMC Plant Biology, ISSN: 1471-2229, Vol: 22, Issue: 1, Page: 366
2022
  • 6
    Citations
  • 0
    Usage
  • 11
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Nitrate is an essential nutrient and an important signaling molecule in plants. However, the molecular mechanisms by which plants perceive nitrate deficiency signaling are still not well understood. Here we report that AtNLP7 protein transport from the nucleus to the cytoplasm in response to nitrate deficiency is dependent on the N-terminal GAF domain. With the deletion of the GAF domain, AtNLP7 always remains in the nucleus regardless of nitrate availability. AtNLP7 also shows reduced activation of nitrate-induced genes due to its impaired binding to the nitrate-responsive cis-element (NRE) as well as decreased growth like nlp7-1 mutant. In addition, AtNLP7 is unable to mediate the reduction of reactive oxygen species (ROS) accumulation upon nitrate treatment. Our investigation shows that the GAF domain of AtNLP7 plays a critical role in the sensing of nitrate deficiency signal and in the nitrate-triggered ROS signaling process.

Bibliographic Details

Wu, Jie; Song, Ying; Zhang, Zi-Sheng; Wang, Jing-Xian; Zhang, Xuan; Zang, Jian-Ye; Bai, Ming-Yi; Yu, Lin-Hui; Xiang, Cheng-Bin

Springer Science and Business Media LLC

Agricultural and Biological Sciences

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know