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Identification of circulating MOG-specific B cells in patients with MOG antibodies

Neurology(R) neuroimmunology & neuroinflammation, ISSN: 2332-7812, Vol: 6, Issue: 6, Page: 625-null
2019
  • 44
    Citations
  • 0
    Usage
  • 59
    Captures
  • 0
    Mentions
  • 76
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    44
  • Captures
    59
  • Social Media
    76
    • Shares, Likes & Comments
      76
      • Facebook
        76

Article Description

OBJECTIVE: To identify circulating myelin oligodendrocyte glycoprotein (MOG)-specific B cells in the blood of patients with MOG antibodies (Abs) and to determine whether circulating MOG-specific B cells are linked to levels and epitope specificity of serum anti-MOG-Abs. METHODS: We compared peripheral blood from 21 patients with MOG-Abs and 26 controls for the presence of MOG-specific B cells. We differentiated blood-derived B cells in vitro in separate culture wells to Ab-producing cells via engagement of Toll-like receptors 7 and 8. We quantified the anti-MOG reactivity with a live cell-based assay by flow cytometry. We determined the recognition of MOG epitopes with a panel of mutated variants of MOG. RESULTS: MOG-Ab-positive patients had a higher frequency of MOG-specific B cells in blood than controls, but MOG-specific B cells were only detected in about 60% of these patients. MOG-specific B cells in blood showed no correlation with anti-MOG Ab levels in serum, neither in the whole group nor in the untreated patients. Epitope analysis of MOG-Abs secreted from MOG-specific B cells cultured in different wells revealed an intraindividual heterogeneity of the anti-MOG autoimmunity. CONCLUSIONS: This study shows that patients with MOG-Abs greatly differ in the abundance of circulating MOG-specific B cells, which are not linked to levels of MOG-Abs in serum suggesting different sources of MOG-Abs. Identification of MOG-specific B cells in blood could be of future relevance for selecting patients with MOG-Abs for B cell-directed therapy.

Bibliographic Details

Winklmeier, Stephan; Schlüter, Miriam; Spadaro, Melania; Thaler, Franziska S; Vural, Atay; Gerhards, Ramona; Macrini, Caterina; Mader, Simone; Kurne, Aslı; Inan, Berin; Karabudak, Rana; Özbay, Feyza Gül; Esendagli, Gunes; Hohlfeld, Reinhard; Kümpfel, Tania; Meinl, Edgar

Ovid Technologies (Wolters Kluwer Health)

Neuroscience; Medicine

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