Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1
Journal of Cell Science, ISSN: 0021-9533, Vol: 123, Issue: 1, Page: 84-94
2010
- 91Citations
- 102Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations91
- Citation Indexes91
- 91
- CrossRef89
- Captures102
- Readers102
- 102
Article Description
The BubR1 checkpoint protein performs multiple functions in mitosis. We have carried out a functional analysis of conserved motifs of human BubR1 (also known as BUB1B) and demonstrate that spindle assembly checkpoint (SAC) and chromosome attachment functions can be uncoupled from each other. Mutation of five proline-directed serine phosphorylation sites, identified in vivo by mass spectrometry, essentially abolishes attachment of chromosomes to the spindle but has no effect on SAC functionality. By contrast, mutation of the two conserved KEN boxes required for SAC function does not impact chromosome congression. Interestingly, the contribution of the two KEN-box motifs is not equal. Cdc20 associates with the N-terminal but not C-terminal KEN box, and mutation of the N-terminal KEN motif results in more severe acceleration of mitotic timing. Moreover, the two KEN motifs are not sufficient for maximal binding of Cdc20 and APC/C, which also requires sequences in the BubR1 C-terminus. Finally, mutation of the GLEBS motif causes loss of Bub3 interaction and mislocalization of BubR1 from the kinetochore; concomitantly, BubR1 phosphorylation as well as SAC activity and chromosome congression are impaired, indicating that the GLEBS motif is strictly required for both major functions of human BubR1.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=72449178000&origin=inward; http://dx.doi.org/10.1242/jcs.056507; http://www.ncbi.nlm.nih.gov/pubmed/20016069; https://journals.biologists.com/jcs/article/123/1/84/30922/Uncoupling-of-the-spindle-checkpoint-and; https://dx.doi.org/10.1242/jcs.056507; https://jcs.biologists.org/content/123/1/84
The Company of Biologists
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