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Cytotoxicity activity of biotransformed ethyl p-methoxycinnamate by Aspergillus niger

Oriental Journal of Chemistry, ISSN: 2231-5039, Vol: 32, Issue: 5, Page: 2731-2734
2016
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Article Description

The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC).

Bibliographic Details

Muhammad Nor Omar; Nor Hazwani Mohd Hasali; Mohd Ambar Yarmo

Oriental Scientific Publishing Company

Chemistry; Biochemistry, Genetics and Molecular Biology; Environmental Science; Pharmacology, Toxicology and Pharmaceutics

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