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Digital holographic microscopy with dual-wavelength phase unwrapping

Applied Optics, ISSN: 1539-4522, Vol: 45, Issue: 3, Page: 451-459
2006
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Article Description

We apply the techniques of digital holography to obtain microscopic three-dimensional images of biological cells. The optical system is capable of microscopic holography with diffraction-limited resolution by projecting a magnified image of a microscopic hologram plane onto a CCD plane. Two-wavelength phase-imaging digital holography is applied to produce unwrapped phase images of biological cells. The method of three-wavelength phase imaging is proposed to extend the axial range and reduce the effect of phase noise. These results demonstrate the effectiveness of digital holography in high-resolution biological microscopy. © 2006 Optical Society of America.

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