Simultaneous determination of six major constituents in the stems of Kadsura heteroclita by LC-DAD
Chromatographia, ISSN: 0009-5893, Vol: 64, Issue: 5-6, Page: 297-302
2006
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Article Description
The stem of Kadsura heteroclita is a traditional Chinese medicine with a variety of biological activities. For efficient monitoring of the quality of the herb, a simple, rapid, and accurate HPLC method has been developed for simultaneous determination of five cyclolanostane triterpenoid compounds (schisanlactone E, nigranoic acid, schisandronic acid, and heteroclitalactones A and D) and one tetrahydrofuran lignan (d-epigalbacin) in the stems of K. heteroclita. These six compounds were separated on a C column by elution with a mobile-phase gradient prepared from acetonitrile and 0.05% (v/v) aqueous phosphoric acid. The flow rate was 1.0 mL min and the detection wavelength was 210 nm. The recovery of the method was in the range 95.03-102.19% and a good linear relationship (r > 0.9999) was obtained between response and compound concentration over a relatively wide range. The method was successfully applied to analysis of the herb collected at different stages of growth. © 2006 Friedr. Vieweg & Sohn/GWV Fachverlage GmbH.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33748785186&origin=inward; http://dx.doi.org/10.1365/s10337-006-0031-7; http://link.springer.com/10.1365/s10337-006-0031-7; https://dx.doi.org/10.1365/s10337-006-0031-7; https://link.springer.com/article/10.1365/s10337-006-0031-7; https://link.springer.com/article/10.1365%2Fs10337-006-0031-7; http://www.springerlink.com/index/10.1365/s10337-006-0031-7
Springer Science and Business Media LLC
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