Differential expression of type III effector BteA protein due to IS481 insertion in Bordetella pertussis
PLoS ONE, ISSN: 1932-6203, Vol: 6, Issue: 3, Page: e17797
2011
- 33Citations
- 44Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations33
- Citation Indexes33
- 33
- CrossRef28
- Captures44
- Readers44
- 44
Article Description
Background: Bordetella pertussis is the primary etiologic agent of the disease pertussis. Universal immunization programs have contributed to a significant reduction in morbidity and mortality of pertussis; however, incidence of the disease, especially in adolescents and adults, has increased in several countries despite high vaccination coverage. During the last three decades, strains of Bordetella pertussis in circulation have shifted from the vaccine-type to the nonvaccine-type in many countries. A comparative proteomic analysis of the strains was performed to identify protein(s) involved in the type shift. Methodology/Principal Finding: Proteomic analysis identified one differentially expressed protein in the B. pertussis strains: the type III cytotoxic effector protein BteA, which is responsible for host cell death in Bordetella bronchiseptica infections. Immunoblot analysis confirmed the prominent expression of BteA protein in the nonvaccine-type strains but not in the vaccine-type strains. Sequence analysis of the vaccine-type strains revealed an IS481 insertion in the 5′ untranslated region of bteA, -136 bp upstream of the bteA start codon. A high level of bteA transcripts from the IS481 promoter was detected in the vaccine-type strains, indicating that the transcript might be an untranslatable form. Furthermore, BteA mutant studies demonstrated that BteA expression in the vaccine-type strains is down-regulated by the IS481 insertion. Conclusion/Significance: The cytotoxic effector BteA protein is expressed at higher levels in B. pertussis nonvaccine-type strains than in vaccine-type strains. This type-dependent expression is due to an insertion of IS481 in B. pertussis clinical strains, suggesting that augmented expression of BteA protein might play a key role in the type shift of B. pertussis. © 2011 Han et al.
Bibliographic Details
10.1371/journal.pone.0017797; 10.1371/journal.pone.0017797.g002; 10.1371/journal.pone.0017797.g006; 10.1371/journal.pone.0017797.g003; 10.1371/journal.pone.0017797.g005; 10.1371/journal.pone.0017797.g001; 10.1371/journal.pone.0017797.g004
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79952514269&origin=inward; http://dx.doi.org/10.1371/journal.pone.0017797; http://www.ncbi.nlm.nih.gov/pubmed/21423776; https://dx.plos.org/10.1371/journal.pone.0017797; https://dx.plos.org/10.1371/journal.pone.0017797.g002; http://dx.doi.org/10.1371/journal.pone.0017797.g002; https://dx.plos.org/10.1371/journal.pone.0017797.g006; http://dx.doi.org/10.1371/journal.pone.0017797.g006; http://dx.plos.org/10.1371/journal.pone.0017797.g003; http://dx.doi.org/10.1371/journal.pone.0017797.g003; https://dx.plos.org/10.1371/journal.pone.0017797.g005; http://dx.doi.org/10.1371/journal.pone.0017797.g005; https://dx.plos.org/10.1371/journal.pone.0017797.g001; http://dx.doi.org/10.1371/journal.pone.0017797.g001; https://dx.plos.org/10.1371/journal.pone.0017797.g004; http://dx.doi.org/10.1371/journal.pone.0017797.g004; https://dx.doi.org/10.1371/journal.pone.0017797; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0017797; https://dx.doi.org/10.1371/journal.pone.0017797.g006; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g006; https://dx.doi.org/10.1371/journal.pone.0017797.g001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g001; https://dx.doi.org/10.1371/journal.pone.0017797.g003; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g003; https://dx.doi.org/10.1371/journal.pone.0017797.g002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g002; https://dx.doi.org/10.1371/journal.pone.0017797.g004; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g004; https://dx.doi.org/10.1371/journal.pone.0017797.g005; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0017797.g005; http://dx.plos.org/10.1371/journal.pone.0017797.g001; http://dx.plos.org/10.1371/journal.pone.0017797.g006; http://www.plosone.org/article/metrics/info:doi/10.1371/journal.pone.0017797; http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017797&type=printable; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0017797; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0017797&type=printable; http://dx.plos.org/10.1371/journal.pone.0017797; http://dx.plos.org/10.1371/journal.pone.0017797.g005; http://dx.plos.org/10.1371/journal.pone.0017797.g002; http://dx.plos.org/10.1371/journal.pone.0017797.g004; http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0017797
Public Library of Science (PLoS)
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know