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NT-ProBNP Levels in Saliva and Its Clinical Relevance to Heart Failure

PLoS ONE, ISSN: 1932-6203, Vol: 7, Issue: 10, Page: e48452
2012
  • 60
    Citations
  • 0
    Usage
  • 70
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    60
    • Citation Indexes
      56
    • Policy Citations
      3
      • Policy Citation
        3
    • Patent Family Citations
      1
      • Patent Families
        1
  • Captures
    70

Article Description

Background: Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter-individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body's health and well being and ~20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non-invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels. Methods: Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at -80°C until analysis. A customised homogeneous sandwich AlphaLISA immunoassay was used to quantify NT-proBNP levels in saliva. Results: Our NT-proBNP immunoassay was validated against a commercial Roche assay on plasma samples collected from HF patients (n = 37) and the correlation was r = 0.78 (p<0.01, y = 1.705× +1910.8). The median salivary NT-proBNP levels in the healthy and HF participants were <16 pg/mL and 76.8 pg/mL, respectively. The salivary NT-proBNP immunoassay showed a clinical sensitivity of 82.2% and specificity of 100%, positive predictive value of 100% and negative predictive value of 83.3%, with an overall diagnostic accuracy of 90.6%. Conclusion: We have firstly demonstrated that NT-proBNP can be detected in saliva and that the levels were higher in heart failure patients compared with healthy control subjects. Further studies will be needed to demonstrate the clinical relevance of salivary NT-proBNP in unselected, previously undiagnosed populations. © 2012 Foo et al.

Bibliographic Details

http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84868319875&origin=inward; http://dx.doi.org/10.1371/journal.pone.0048452; http://www.ncbi.nlm.nih.gov/pubmed/23119023; https://dx.plos.org/10.1371/journal.pone.0048452.t002; http://dx.doi.org/10.1371/journal.pone.0048452.t002; https://dx.plos.org/10.1371/journal.pone.0048452; https://dx.plos.org/10.1371/journal.pone.0048452.g001; http://dx.doi.org/10.1371/journal.pone.0048452.g001; https://dx.plos.org/10.1371/journal.pone.0048452.t001; http://dx.doi.org/10.1371/journal.pone.0048452.t001; https://dx.plos.org/10.1371/journal.pone.0048452.g002; http://dx.doi.org/10.1371/journal.pone.0048452.g002; https://dx.doi.org/10.1371/journal.pone.0048452.g001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0048452.g001; https://dx.doi.org/10.1371/journal.pone.0048452.t002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0048452.t002; https://dx.doi.org/10.1371/journal.pone.0048452; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0048452; https://dx.doi.org/10.1371/journal.pone.0048452.t001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0048452.t001; https://dx.doi.org/10.1371/journal.pone.0048452.g002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0048452.g002; http://dx.plos.org/10.1371/journal.pone.0048452.g002; http://www.plosone.org/article/metrics/info:doi/10.1371/journal.pone.0048452; http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0048452&type=printable; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0048452; http://dx.plos.org/10.1371/journal.pone.0048452.t001; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0048452&type=printable; http://dx.plos.org/10.1371/journal.pone.0048452; http://dx.plos.org/10.1371/journal.pone.0048452.g001; http://dx.plos.org/10.1371/journal.pone.0048452.t002; http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0048452

Jared Yong Yang Foo; Yunxia Wan; Karam Kostner; Alicia Arivalagan; John Atherton; Justin Cooper-White; Goce Dimeski; Chamindie Punyadeera; Ali J. Marian

Public Library of Science (PLoS)

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Multidisciplinary

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