Pyruvate kinase M2 and lactate dehydrogenase a are overexpressed in pancreatic cancer and correlate with poor outcome
PLoS ONE, ISSN: 1932-6203, Vol: 11, Issue: 3, Page: e0151635
2016
- 68Citations
- 78Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations68
- Citation Indexes68
- 68
- CrossRef27
- Captures78
- Readers78
- 78
Article Description
Pancreatic cancer has a 5-year survival rate of less than 4%. Despite advances in diagnostic technology, pancreatic cancer continues to be diagnosed at a late and incurable stage. Accurate biomarkers for early diagnosis and to predict treatment response are urgently needed. Since alteration of glucose metabolism is one of the hallmarks of cancer cells, we proposed that pyruvate kinase type M2 (M2PK) and lactate dehydrogenase A (LDHA) enzymes could represent novel diagnostic markers and potential therapeutic targets in pancreatic cancer. In 266 tissue sections from normal pancreas, pancreatic cystic neoplasms, pancreatic intraepithelial neoplasia (PanIN) and cancer, we evaluated the expression of PKM2, LDHA, Ki-67 and CD8+ by immunohistochemistry and correlated these markers with clinicopathological characteristics and patient survival. PKM2 and LDHA expression was also assessed by Western blot in 10 human pancreatic cancer cell lines. PKM2 expression increased progressively from cyst through PanIN to cancer, whereas LDHA was over-expressed throughout the carcinogenic process. All but one cell line showed high expression of both proteins. Patients with strong PKM2 and LDHA expression had significantly worse survival than those with weak PKM2 and/or LDHA expression (7.0 months vs. 27.9 months, respectively, p = 0.003, log rank test). The expression of both PKM2 and LDHA correlated directly with Ki-67 expression, and inversely with intratumoral CD8+ cell count. PKM2 was significantly overexpressed in poorly differentiated tumours and both PKM2 and LDHA were overexpressed in larger tumours. Multivariable analysis showed that combined expression of PKM2 and LDHA was an independent poor prognostic marker for survival. In conclusion, our results demonstrate a high expression pattern of two major glycolytic enzymes during pancreatic carcinogenesis, with increased expression in aggressive tumours and a significant adverse effect on survival.
Bibliographic Details
10.1371/journal.pone.0151635; 10.1371/journal.pone.0151635.g005; 10.1371/journal.pone.0151635.t001; 10.1371/journal.pone.0151635.g002; 10.1371/journal.pone.0151635.g007; 10.1371/journal.pone.0151635.g001; 10.1371/journal.pone.0151635.g003; 10.1371/journal.pone.0151635.g006; 10.1371/journal.pone.0151635.g004
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84962176550&origin=inward; http://dx.doi.org/10.1371/journal.pone.0151635; http://www.ncbi.nlm.nih.gov/pubmed/26989901; https://dx.plos.org/10.1371/journal.pone.0151635.g005; http://dx.doi.org/10.1371/journal.pone.0151635.g005; https://dx.plos.org/10.1371/journal.pone.0151635.t001; http://dx.doi.org/10.1371/journal.pone.0151635.t001; https://dx.plos.org/10.1371/journal.pone.0151635.g002; http://dx.doi.org/10.1371/journal.pone.0151635.g002; https://dx.plos.org/10.1371/journal.pone.0151635.g007; http://dx.doi.org/10.1371/journal.pone.0151635.g007; https://dx.plos.org/10.1371/journal.pone.0151635; https://dx.plos.org/10.1371/journal.pone.0151635.g001; http://dx.doi.org/10.1371/journal.pone.0151635.g001; https://dx.plos.org/10.1371/journal.pone.0151635.g003; http://dx.doi.org/10.1371/journal.pone.0151635.g003; https://dx.plos.org/10.1371/journal.pone.0151635.g006; http://dx.doi.org/10.1371/journal.pone.0151635.g006; https://dx.plos.org/10.1371/journal.pone.0151635.g004; http://dx.doi.org/10.1371/journal.pone.0151635.g004; https://dx.doi.org/10.1371/journal.pone.0151635.t001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.t001; https://dx.doi.org/10.1371/journal.pone.0151635.g004; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g004; https://dx.doi.org/10.1371/journal.pone.0151635.g003; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g003; https://dx.doi.org/10.1371/journal.pone.0151635.g006; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g006; https://dx.doi.org/10.1371/journal.pone.0151635.g002; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g002; https://dx.doi.org/10.1371/journal.pone.0151635; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151635; https://dx.doi.org/10.1371/journal.pone.0151635.g005; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g005; https://dx.doi.org/10.1371/journal.pone.0151635.g007; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g007; https://dx.doi.org/10.1371/journal.pone.0151635.g001; https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0151635.g001; http://dx.plos.org/10.1371/journal.pone.0151635.g005; http://dx.plos.org/10.1371/journal.pone.0151635.g006; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0151635&type=printable; http://dx.plos.org/10.1371/journal.pone.0151635; http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151635; http://dx.plos.org/10.1371/journal.pone.0151635.t001; http://dx.plos.org/10.1371/journal.pone.0151635.g003; http://www.plosone.org/article/metrics/info:doi/10.1371/journal.pone.0151635; http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0151635&type=printable; http://dx.plos.org/10.1371/journal.pone.0151635.g002; http://dx.plos.org/10.1371/journal.pone.0151635.g004; http://dx.plos.org/10.1371/journal.pone.0151635.g001; http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0151635; http://dx.plos.org/10.1371/journal.pone.0151635.g007
Public Library of Science (PLoS)
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know