Novel anti-PTEN C2 domain monoclonal antibodies to analyse the expression and function of PTEN isoform variants
PLoS ONE, ISSN: 1932-6203, Vol: 18, Issue: 8 August, Page: e0289369
2023
- 2Citations
- 6Captures
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Metrics Details
- Citations2
- Citation Indexes2
- Captures6
- Readers6
Article Description
PTEN is a major tumor suppressor gene frequently mutated in human tumors, and germline PTEN gene mutations are the molecular diagnostic of PTEN Hamartoma Tumor Syndrome (PHTS), a heterogeneous disorder that manifests with multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. A diversity of translational and splicing PTEN isoforms exist, as well as PTEN C-terminal truncated variants generated by disease-associated nonsense mutations. However, most of the available anti-PTEN monoclonal antibodies (mAb) recognize epitopes at the PTEN C-terminal tail, which may introduce a bias in the analysis of the expression of PTEN isoforms and variants. We here describe the generation and precise characterization of anti-PTEN mAb recognizing the PTEN C2-domain, and their use to monitor the expression and function of PTEN isoforms and PTEN missense and nonsense mutations associated to disease. These anti-PTEN C2 domain mAb are suitable to study the pathogenicity of PTEN C-terminal truncations that retain stability and function but have lost the PTEN C-terminal epitopes. The use of well-defined anti-PTEN mAb recognizing distinct PTEN regions, as the ones here described, will help to understand the deleterious effects of specific PTEN mutations in human disease.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85166171641&origin=inward; http://dx.doi.org/10.1371/journal.pone.0289369; http://www.ncbi.nlm.nih.gov/pubmed/37527256; https://dx.plos.org/10.1371/journal.pone.0289369; https://dx.doi.org/10.1371/journal.pone.0289369; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0289369
Public Library of Science (PLoS)
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