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Cytological detection of male recombination in Drosophila willistoni

Cytologia, ISSN: 0011-4545, Vol: 69, Issue: 4, Page: 359-365
2004
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Article Description

We record the occurrence of male recombination, detected in cytological preparations of testes in 2 Drosophila willistoni population samples. Comparative analysis of meiotic figures in imaginal discs of testes and the salivary gland polytene chromosomes of male third instar larvae were employed. We observed anaphase bridges, suggesting the occurrence of chiasmata and fragmented chromatids involving the second chromosomal pair and the heterozygous inversions IILF and IILD+E segregating in the same individuals. This was observed in non-stressed larvae maintained at physiological temperature, and opens a wide field to study the factors that regulate crossing-over in natural populations of the highly polymorphic D. willistoni. We also observed what appears to be crossing-over in the tip of chromosomal arm IIL, in a sole male heterozygote for the inversion IILH of the G3 natural population. © 2004 The Japan Mendel Society.

Bibliographic Details

Marisa Conceição Dos Santos-Colares; Tiago Hoerbe Degrandi; Vera Lúcia S. Valente

International Society of Cytology

Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology

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