A FRET-Based Calcium Biosensor with Fast Signal Kinetics and High Fluorescence Change
Biophysical Journal, ISSN: 0006-3495, Vol: 90, Issue: 5, Page: 1790-1796
2006
- 256Citations
- 341Captures
- 2Mentions
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations256
- Citation Indexes255
- 255
- CrossRef240
- Patent Family Citations1
- Patent Families1
- Captures341
- Readers341
- 341
- Mentions2
- References2
- Wikipedia2
Article Description
Genetically encoded calcium biosensors have become valuable tools in cell biology and neuroscience, but some aspects such as signal strength and response kinetics still need improvement. Here we report the generation of a FRET-based calcium biosensor employing troponin C as calcium-binding moiety that is fast, is stable in imaging experiments, and shows a significantly enhanced fluorescence change. These improvements were achieved by engineering magnesium and calcium-binding properties within the C-terminal lobe of troponin C and by the incorporation of circularly permuted variants of the green fluorescent protein. This sensor named TN-XL shows a maximum fractional fluorescence change of 400% in its emission ratio and linear response properties over an expanded calcium regime. When imaged in vivo at presynaptic motoneuron terminals of transgenic fruit flies, TN-XL exhibits highly reproducible fluorescence signals with the fastest rise and decay times of all calcium biosensors known so far.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0006349506723657; http://dx.doi.org/10.1529/biophysj.105.073536; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33646145526&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/16339891; https://linkinghub.elsevier.com/retrieve/pii/S0006349506723657
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know