Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice
Brazilian Journal of Medical and Biological Research, ISSN: 1414-431X, Vol: 32, Issue: 11, Page: 1429-1436
1999
- 6Citations
- 7,590Usage
- 26Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations6
- Citation Indexes6
- CrossRef5
- Usage7,590
- Full Text Views6,907
- 6,907
- Abstract Views683
- 683
- Captures26
- Readers26
- 26
Article Description
Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57B1/6 mice. C57B1/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-γ-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in the peritoneal exudates. The administration of Asc extract increased NADPH diaphorase activity (2 days: control = 0, BCG = 7%, BCG + Asc = 13%, and Asc = 4%; 7 days: control = 4, BCG = 13%, BCG + Asc = 21%, and Asc = 4.5%) and TNF-α levels (mean ± D; 2 days: control = 0, BCG = 169 ± 13, BCG + Asc = 202 ± 37, and Asc = 0; 7 days: control = 0, BCG = 545 ± 15.5, BCG + Asc = 2206 ± 160.6, and ASC = 126 ± 26; 14 days: control = 10 ± 1.45, BCG = 9 ± 1.15, BCG + Asc = 126 ± 18, and Asc = 880 ± 47.67 pg/ml) in the early phase of BCG infection. Low levels of NO production were detected at 2 and 7 days after BCG infection, increasing at 14 days (mean ± SD; 2 days: control = 0, BCG = 3.7 ± 1.59, BCG + Asc = 0.82 ± 0.005, Asc = 0.48 ± 0.33; 7 days: control = 0, BCG = 2.78 ± 1.54, BCG + Asc = 3.07 ± 1.05, Asc = 0; 14 days: control = 0, BCG = 9.05 ± 0.53, BCG + Asc = 9.61 ± 0.81, ASC = 10.5 ± 0.2 (2 × 10) cells/ml). Furthermore, we also observed that Asc co-injection induced a decrease of BCG-colony-forming units (CFU) in the spleens of BCG-infected mice during the first week of infection (mean ± SD; 2 days: BCG = 1.13 ± 0.07 and BCG + Asc = 0.798 ± 0.305; 7 days: BCG = 1.375 ± 0.194 and BCG + Asc = 0.548 ± 0.0226; 14 days: BCG = 0.473 ± 0.184 and BCG + Asc = 0.675 ± 0.065 (× 10) CFU). The present data suggest that Asc induces the enhancement of the immune response in the early phase of BCG infection.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032763642&origin=inward; http://dx.doi.org/10.1590/s0100-879x1999001100014; http://www.ncbi.nlm.nih.gov/pubmed/10559845; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100014&lng=en&tlng=en; http://www.scielo.br/pdf/bjmbr/v32n11/3604c.pdf; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X1999001100014&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100014; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X1999001100014; https://dx.doi.org/10.1590/s0100-879x1999001100014; https://www.scielo.br/j/bjmbr/a/FnK5QDbfCzJTTw4bCRGF8Bt/?lang=en
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