Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures
Brazilian Archives of Biology and Technology, ISSN: 1678-4324, Vol: 58, Issue: 6, Page: 923-928
2015
- 5Citations
- 2,293Usage
- 25Captures
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Article Description
Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x10 particles.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84952661076&origin=inward; http://dx.doi.org/10.1590/s1516-89132015060367; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923&lng=en&tlng=en; http://www.scielo.br/pdf/babt/v58n6/1516-8913-babt-58-06-00923.pdf; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132015000600923&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132015000600923; https://dx.doi.org/10.1590/s1516-89132015060367; https://www.scielo.br/j/babt/a/hc7ByTYPNgh8BWRvtVQGzcg/?lang=en
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