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Development of a membrane-based microwave-mediated electrochemical ELISA method for TNF-α detection in patients with periodontitis

Analytical Sciences, ISSN: 1348-2246, Vol: 29, Issue: 9, Page: 927-930
2013
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Article Description

The tumor necrosis factor-α (TNF-α) is implicated in periodontal disease, and there was an attempt to quantitate it by a membrane-based microwave-mediated electrochemical enzyme-linked immunosorbent assay (MMeELISA) using p-aminophenyl phosphate (pAPP) with an over-all time of 1.5 h. A differential pulse voltammetric signal increased linearly with an increase in the amount of TNF-α with a detection limit of 0.48 pg mL. This bio-sensing platform revealed a significant difference in the TNF-α level between GCF samples from periodontal patients and healthy volunteers. © 2013 The Japan Society for Analytical Chemistry.

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