Biphasic oxygen tension promotes the formation of transferable blastocysts in patients without euploid embryos in previous monophasic oxygen cycles

This study evaluated whether the concentration of biphasic O (5–2%) promotes the formation of qualified blastocysts (QBs) and euploid blastocysts and increases the probability of cycles with transferable blastocysts. Total 90 patients (180 cycles) without euploid blastocysts in previous monophasic O (5%) cycles were enrolled for an additional cycle of biphasic O (5–2%). In the biphasic O (5–2%) tension group, the rate of QB (35.8%, 225/628) were significantly higher than those in the monophasic O (5%) group (23.5%, 137/582; p < 0.001). In addition, the number of euploid blastocysts (0.5 ± 0.8) and the percentage of cycles with transferable blastocysts were significantly higher in the biphasic O (5–2%) group (57.8%, 52/90) than in the monophasic O (5%) group (0 and 35.6%, 32/90, respectively; p = 0.003). Multivariable regression analysis also indicated that the QB rate and the probability of cycles with transferable blastocysts correlated with O tension (OR: 1.535, 95% CI: 1.325–1.777, and OR: 3.191, 95% CI: 1.638–5.679, respectively; p < 0.001). Biphasic O culture can be used as an alternative strategy to increase the number of euploid QBs and the probability of cycles with transferable blastocysts in patients with a poor prognosis.