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Bridging the Research Gap: Establishing a Rabbit Model for Perianal Fistula (Nassor-E Maqad) Study

Research Square
2024
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Article Description

Fistula-in-ano, termed ‘Nassor-e-Maqad’in traditional Arabic medicine, presents a challenging perianal pathology necessitating a robust experimental framework for comprehensive inquiry. This investigation endeavors to establish an induced Fistula-in-ano model in New Zealand White (NZW) Rabbits (n=12) utilizing a protocol involving surgical suturing steel wire implantation for durations of 20 days (Group ‘A’) and 40 days (Group ‘B'). The principal aim of this research is to develop an induced Fistula-in-anomodel in NZW rabbits, with the objective of enhancing comprehension of perianal disease pathogenesis and exploring novel therapeutic modalities. Given the absence of universally efficacious and complication-free treatments, the development of innovative therapeutic approaches is imperative. Hence, the creation of an optimal animal model capable of simulating persistent anal fistulas with lesions resembling clinical manifestations is vital for preclinical investigations in this domain. Upon imaging (X-ray and perianal ultrasonography) the patency of fistula was evaluated whereas histological evaluation exhibited that animals in Group ‘A’ (n=06) fistula has openings with modest epithelialization subsequent to the removal of the surgical steel suture ring, along with favorable diagnostic outcomes, 20 days post-surgery. Conversely, animals in Group ‘B’ (n=6), upon steel wire removal after 40 days, displayed notable changes in epithelization, indicative of further progression in the induced fistula model. In summary, this study successfully establishes a preclinical rabbit model of perianal fistula devoid of chemical agents, relying solely on surgical induction via surgical suturing steel wire (SSSW). The considerable anatomical and histological parallels between rabbit and human perianal pathophysiology underscore the suitability of rabbits as an experimental model for advancing therapeutic strategies in proctology.

Bibliographic Details

Syed Abdul Shakoor Bukhari; Zaibunnisa Begum; Pradeep B. Patil

Research Square Platform LLC

Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Medicine; Neuroscience; Psychology; Dentistry

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