Investigating the effect of mouthwash containing chitosan / magnesium oxide / silver nanocomposite on Helicobacter pylori

Background: Helicobacter pylori is known to be the main cause of stomach cancer and may be transmitted through the mouth or feces. Therefore, this study was conducted with the aim of preparing a mouthwash consisting of chitosan / magnesium oxide, and silver nanocomposite against Helicobacter. Methods: The new nanocomposite group was used as a test group, and five conventional antibiotic groups were used to compare the antimicrobial effects of the new material as a positive control and distilled water as a negative control. The two-way ANOVA test was used, and the data was analyzed by SPSS software version 24. Results: The results showed that chitosan, magnesium oxide, and silver nanocomposite mouthwash in concentrations of 50, 25, 12.5, and 6.25 micrograms/ml have antibacterial effects against Helicobacter pylori. The two-way ANOVA analysis test showed that among all the substances tested, metronidazole had the largest lack of growth halo, so that this lack of growth halo had a significant difference compared to the rest of the groups (P<0.0001). Also, the lowest number of non-growth auras was related to the tested nanocomposite sample, which was statistically significantly different from all groups except gentamicin and amikacin (P<0.0001). Conclusions: The results of the present study showed that the composition prepared in this study has significant effects in reducing Helicobacter pylori.