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Identification of Distinct Immune Cells Associated with Various Clinical Presentations of COVID-19

SSRN Electronic Journal
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Metric Options:   Counts1 Year3 Year

Metrics Details

  • Usage
    753
    • Abstract Views
      732
    • Downloads
      21
  • Captures
    1
    • Readers
      1
      • SSRN
        1

Article Description

The cell-mediated protective and pathogenic immune responses to SARS-CoV-2 infection remain largely unknown. Here, we identified 76 distinct cell subsets in the PBMC samples that were associated with various clinical presentations of COVID-19 using scRNA-seq technology coupled with a deep and comprehensive analysis of unique cell surface markers and differentially expressed genes. Notable cell subsets include (NCAM1hiCD160+)NK and (TRAV1-2+CD8+)MAIT cells increased in the asymptomatic subjects, (LAG3+CD160+CD8+)NKT cells increased in the symptomatic patients, (CD68-CSF1R-IL1BhiCD14+)classical monocytes associated with age and disease severity, (CD33-HLA-DMA-CD14+)classical monocytes and (CLEC10A-S100A9lo)pDC associated with the viral persistence, neutrophils and (CD68-CSF1R-IL1BhiCD14+ )classical monocytes dramatically increased in a fatal patient, whereas (LAG3+CD160+CD8+)NKT and (FOXP3+IL2RA+IL7R+CD4+)Treg cells markedly increased in a patient with humoral immunodeficiency. The GO and KEGG analyses identified enriched pathways related to immune responses, inflammation, apoptosis and other processes. These findings may enhance our understanding of the immunopathogenesis of COVID-19 and help develop novel strategies against SARS-CoV-2 infection. Funding Information: This study is supported in part by the Department of Science and Technology of Shaanxi Province (Grant No. 2020ZDXM2-SF-02) (CZ and BS) and the operational funds from The First Affiliated Hospital of Xi’an Jiaotong University (CZ and BS). Declaration of Interests: The authors declare that they have no competing financial interests.

Bibliographic Details

Xiaorui Wang; Han Bai; Junpeng Ma; Hongyu Qin; Tingting Jiang; Weikang Mao; Qiqi Zeng; Fang Hu; Yan Teng; Lin Fan; Yang Zhao; Xiaobei Chen; Xin Qi; Mengyang Li; Meng Jiang; Jiao Xu; Qindong Shi; Zhihong Shi; Jiajia Ma; Jing Wu; Jianfeng Han; Yankui Wang; Jingcan Hao; Xi Ding; Yue Wang; Yuanrui Liu; Tianlong Huang; Chao Fang; Changli Ge; Dong Li; Ke Hu; Binghong Zhang; Xianwen Ren; Baojun Zhang; Bingyin Shi; Chengsheng Zhang

Elsevier BV

SARS-CoV-2; COVID-19; asymptomatic infection; disease severity; viral persistence; single-cell RNA sequencing (scRNA-seq); PBMCs

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