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Fluorescent Probe-Encapsulated Smart Nanohydrogel to Enhance Sensitivity Toward Hydrogen Peroxide in Living Cells

SSRN, ISSN: 1556-5068
2022
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  • Usage
    170
    • Abstract Views
      144
    • Downloads
      26

Article Description

Hydrogen peroxide (H 2 O 2 ) is a crucial biomarker in cellular signal transduction. Sensing of endogenous H 2 O 2 is of great significance for the prevention and diagnosis of diseases. Hydrophobic small-molecule fluorescent probes for the H 2 O 2 -sensing generally suffered from low water solubility and high trend to aggregate, resulting in poor cell-membrane permeability and unsatisfactory sensitivity. To overcome this problem, a nanohydrogel-encapsulation strategy for the fluorescent probes is proposed here. As a proof of concept, a fluorescent probe-encapsulated reduction-sensitive smart nanohydrogel was fabricated as a new nanosensor. The nanohydrogel, which was prepared using cystinamide derivatives, can encapsulate and deliver a large number of hydrophobic fluorescent probe molecules into living HeLa cells. In the cytoplasm, the nanohydrogels were reduced and degraded by glutathione, and the fluorescent probe molecules released from the nanohydrogel were enriched and concentrated in some local regions, resulting in enhanced imaging of endogenous H 2 O 2 . This general and facile nanohydrogel-assisted strategy is applicable to multiple fluorescent probes, thus enabling imaging of diverse endogenous bioactive molecules.

Bibliographic Details

Jun Liu; Chen Chang; Qingyue Liu; Quanda Yu; Shiyu Wang; Mingzhe Z. Liu; Huizi Man; Yi Xiao; Peng Yang

Elsevier BV

Multidisciplinary; hydrogen peroxide; nanohydrogels; fluorescent probe; reduction-sensitive

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