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Imprinted Polymer Beads Featuring Both Predefined Multiple-Point Interaction and Accessible Binding Sites for Precise Recognition of 2’-Deoxyadenosine

SSRN, ISSN: 1556-5068
2022
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  • Usage
    192
    • Abstract Views
      163
    • Downloads
      29

Article Description

“Tailor-made” formation of complementary binding sites via lock-and-key molecular binding mechanism is critical for selective adsorption of target molecules in environmental and chemical engineering fields. However, an innovative design of a molecularly imprinted polymers (MIPs) featuring both predefined multiple-point interaction and accessible binding sites still remains a challenging task. Herein, MIPs beads (PC-MIPs) consisting of photo-crosslinked functional polymer building blocks from methacrylic acid (MAA), 1-(vinylbenzyl) thymidine (VBT) and post-crosslinking monomer cinnamoyloxyethyl methacrylate (CEMA) were easily fabricated by emulsion droplet micro-reactors, and then their precise identification of 2’-deoxyadenosine (dA) was studied by batch mode adsorption. PC-MIPs microspheres exhibited surface pores and interconnected chambers, and the internal foam-like framework may be caused by the adequate encapsulation of bubbles from low-boiling separation phase. Due to the high density of accessible imprinted sites and strong interaction with dA, over 67% of dA was captured within the initial 20 h.The maximum monolayer adsorption capacity of PC-MIPs was calculated to be 134.5 μmol g -1 for dA, surpassing most of the reported dA-imprinted sorbents. High-affinity sites from Scatchard analysis accounted for about 64.08% of total binding sites, suggesting the synergy of predefined multiple-point interaction from double monomers and imprinting effect. In addition, PC-MIPs also showed good selectivity (imprinting factor above 2.71) and high stability, as well as excellent recyclability toward dA. Moreover, 82.4% of dA was extracted by PC-MIPs from spiked human urine samples, owing to the advantage of post-crosslinking strategy enabling more stable interaction between the template molecules and functional polymers. Therefore, our work demonstrated an alternate strategy to design polymers bearing precise molecular recognition capability, and provided a new perspective for dA enrichment from biological samples.

Bibliographic Details

Jianming Pan; Zhuangxin Wei; Pan Wang; Xiaohua Tian

Elsevier BV

Multidisciplinary; Post-crosslinking imprinted polymers (PC-MIPs); Predefined multiple-point interaction; Emulsion droplet micro-reactors; Accessible binding sites; Selective adsorption 2'-deoxyadenosine

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