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Fast Single-Layer Reconstruction for Three-Dimensional Structured Illumination Microscopy

SSRN, ISSN: 1556-5068
2022
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    Citations
  • 241
    Usage
  • 1
    Captures
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    Mentions
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Metric Options:   Counts1 Year3 Year

Metrics Details

  • Usage
    241
    • Abstract Views
      187
    • Downloads
      54
  • Captures
    1
  • Ratings
    • Download Rank
      765,790

Article Description

Although three-dimensional structured illumination microscopy (3D-SIM) that enhances the spatial resolution of widefield microscope in three dimensions has been successfully commercialized as a variety of products, the low imaging speed impedes its application in fast subcellular dynamics. To adapt to fast dynamics, most 3D-SIM setups provide an option for the users by canceling the axial scanning during the acquisition, by which two-dimensional optically-sectioned, super-resolved (OS-SR) time series can be obtained with single-layer 3D-SIM data. However, the conventional reconstruction workflow implemented in the frequency domain generally takes seconds for every single reconstruction, which nullifies real-time feedback for these 3D-SIM setups. In this paper, we establish a fast single-layer reconstruction approach for 3D-SIM by extending a high-speed reconstruction framework, joint space-frequency reconstruction (JSFR), to single-layer 3D-SIM. Consequently, the reconstruction speed is improved by 90-fold over the conventional method, without compromising the image quality. This approach is expected to provide a useful tool for 3D-SIM microscope manufacturers and their users to achieve real-time, single-layer imaging of living cells.

Bibliographic Details

Ming Lei; Zhaojun Wang; Tianyu Zhao; Yansheng Liang; Shaowei Wang; Jingxiang Zhang; Yanan Cai; Tongsheng Chen

Elsevier BV

Multidisciplinary; single-layer reconstruction; three-dimensional super-resolution microscopy; structured illumination microscopy; real-time imaging; living-cell imaging

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