Fast Single-Layer Reconstruction for Three-Dimensional Structured Illumination Microscopy
SSRN, ISSN: 1556-5068
2022
- 241Usage
- 1Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Although three-dimensional structured illumination microscopy (3D-SIM) that enhances the spatial resolution of widefield microscope in three dimensions has been successfully commercialized as a variety of products, the low imaging speed impedes its application in fast subcellular dynamics. To adapt to fast dynamics, most 3D-SIM setups provide an option for the users by canceling the axial scanning during the acquisition, by which two-dimensional optically-sectioned, super-resolved (OS-SR) time series can be obtained with single-layer 3D-SIM data. However, the conventional reconstruction workflow implemented in the frequency domain generally takes seconds for every single reconstruction, which nullifies real-time feedback for these 3D-SIM setups. In this paper, we establish a fast single-layer reconstruction approach for 3D-SIM by extending a high-speed reconstruction framework, joint space-frequency reconstruction (JSFR), to single-layer 3D-SIM. Consequently, the reconstruction speed is improved by 90-fold over the conventional method, without compromising the image quality. This approach is expected to provide a useful tool for 3D-SIM microscope manufacturers and their users to achieve real-time, single-layer imaging of living cells.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85179573304&origin=inward; http://dx.doi.org/10.2139/ssrn.4174217; https://www.ssrn.com/abstract=4174217; https://dx.doi.org/10.2139/ssrn.4174217; https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4174217; https://ssrn.com/abstract=4174217
Elsevier BV
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