Role of SIRT5 in Analgesic Effectiveness of Moxibustion at ST36 in Inflammatory Pain Mice
SSRN Electronic Journal
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Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
SIRT5 is a significant molecular participating in the pathology process of inflammatory diseases. To investigate the impact of SIRT5 on analgesic effectiveness of moxibustion, mice with complete Freund’s adjuvant- (CFA-) induced inflammatory pain model was established. Moxibustion were applied at Zusanli (ST36) acupoints of inflammatory pain mice. The analgesic effectiveness was evaluated, respectively, by the thermal withdrawal latency (TWL), the paw withdrawal threshold (PWT), and ELISA assay. SIRT5 expression was tested by immunofluorescence and western blot. The results showed that compared with CFA group, both PWL and TWL increased with moxibustion and MC3482 intervention at ST36. In addition, compared to CFA-induced group, the inflammatory mediators, including IL-1β and TNF-α went down; and in contrast, the anti-inflammatory cytokines, IL-4 and TGF-β with moxibustion and MC3482 ST36 acupoint injection went up. Western blot results showed that decreasing expression of SIRT5 at the ST36 site with moxibustion and MC3482 injection, compared to CFA-induced group. While the SIRT5 expression in the right paw of mice with moxibustion and MC3482 injection increased, compared to CFA-induced group. This work revealed that SIRT5 expression was involved in the moxibustion analgesia might be a potential mediator in regulating analgesia. Funding Information: This work has been supported by grants from the National Natural Science Foundation of China (82105010), Chongqing Postdoctoral Science Foundation (cstc2021jcyj-bshX0124), Acupuncture and Chronobiology Key Laboratory of Sichuan Province Open Project (2021006). Conflict of Interests: The authors declare that they have no conflicts of interest and no financial interests related to the material of this manuscript. Ethical Approval: All animal experiments were conducted following the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals and the ethical guidelines of the International Association for the Study of Pain. All experimental procedures were approved by the Animal Care and Use Committee of Chengdu University of Traditional Chinese Medicine (reference number: AECCDUTCM-2018-11).
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