Synergism Variation between Intracellular Glutathione, Phycocyanin and Sod in Microalgae by Carbon Quantum Dot Fluorescence

Based on the use of CQDs as fluorescent probe and covalent coupling method to detect biological molecules with amino groups, to deeply analysis and detect the metabolism of Microcystis aeruginosa. The metabolic changes of carboxyl biomolecules in Microcystis aeruginosa were analyzed by covalent coupling method, including GSH, phycocyanin and SOD enzyme. The changes of GSH content and its correlation between phycocyanin, SOD were analyzed. The content of phycocyanin and SOD reached the maximum on the 65th day, and GSH was more sensitive to the growth and metabolism of microalgae. GSH plays an important role in reducing the external oxidative damage of microalgae cells. GSH synthesis, GSH/GSSG mutual transformation, synthesis of phytochelating peptides (PC), ASA-GSH cycle and metabolism and other physiological activities have interactions, which play a key role in maintaining the antioxidant properties of microalgae and regulating redox-sensitive signal transduction.