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Construction of Green Fluorescence Carbon Quantum Dots Based on Fluorescent/Colorimetric/ Smartphone-Assisted Multi-Signal Strategy for Visual Detection of ClO- and Cellular Analysis

SSRN, ISSN: 1556-5068
2023
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  • Usage
    181
    • Abstract Views
      146
    • Downloads
      35

Article Description

Hypochlorite and its derivative hypochlorous acid (HClO) are monumental and meaningful redox regulator in reactive oxygen species,which played considerable role in bacterial disinfection and body immunity,protecting the body from outside invaders. However, overabundance of ClO- has also been demonstrated closely related to oxidative stress damage to biomolecules,cardiovascular and inflammatory disease.Hence, accurate and sensitive monitoring of hypochlorite (ClO-) concentration was urgently needed in the fields of life sciences,food and environment. Bright green fluorescent carbon quantum dots (G-CQDs) were synthesized utilizing one-step hydrothermal method with citric acid and acriflavine precursors. Through TEM, FTIR, XPS and zeta potential characterization procedures, G-CQDs illustrated uniformly dispersed and significant number of -NH2 and -OH on the surface. Meanwhile,the dual-mode optical strategy with fluorescence and colorimetric analysis exhibited wide linear range and low detection limit response to ClO-. The fluorescence changes of G-CQDs were identified via smartphone to realize mobile sensing of ClO-. Subsequently, G-CQDs was applied for visualization and quantitative detection of ClO- in drinking water samples with satisfactory recovery rate. More importantly, G-CQDs demonstrated good water solubility, optical stability and excellent biocompatibility,which offered a promising analysis approach in cell imaging and exogenous ClO- detection in living cells. G-CQDs illustrated bright prospect and great potential in practical application of ClO- associated disease prevention and early clinical diagnosis.

Bibliographic Details

Huajie Chen; Dai Li; Yutao Zheng; Kui Wang; He Zhang; Huiyun Wen; Weiming Xue; Saipeng Huang

Elsevier BV

Multidisciplinary; Hypochlorite; Carbon quantum dots; Optical dual-mode; Smartphone-assisted; Cell imaging

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