Injectable, Anti-Collapse, Highly Adhesive and Plasticity Bioactive Artificial Bone Promotes Bone Regeneration by Moderating Oxidative Stress in Osteoporotic Bone Defect

The treatment of osteoporotic bone defect remains a big clinical challenge because osteoporosis (OP) is associated with oxidative stress and high levels of reactive oxygen species (ROS), a condition detrimental for bone formation. Anti-oxidative nanomaterials such as selenium nanoparticles (SeNPs) have positive effect on osteogenesis owing to their pleiotropic pharmacological activity which can exert anti-oxidative stress functions to prevent bone loss and facilitate bone regeneration in OP. In the current study a strategy of one-pot method by introducing Poly (lactic acid-carbonate) (PDT) and β-Tricalcium Phosphate (β-TCP) with SeNPs, is developed to prepare an injectable, anti-collapse, shape-adaptive and highly adhesive artificial bone material (PDT-TCP-SE). The PDT-TCP-SE artificial bone exhibits sufficient adhesion in biological microenvironments and good biocompatibility, osteogenic effect, angiogenic effect and anti-inflammatory as well as anti-oxidative effect in vitro and in vivo. Moreover, the PDT-TCP-SE can protect BMSCs from erastin-induced ferroptosis through the Sirt1/Nrf2/GPX4 antioxidant pathway, which, in together, demonstrated the artificial bone as a novel biomaterial with potential clinical application for the future treatment of osteoporotic bone defects.