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Antimicrobial Susceptibility and Genomic Characterization of Vibrio Parahaemolyticus Isolated from Aquatic Foods in 15 Provinces, China, 2020

SSRN, ISSN: 1556-5068
2024
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  • Usage
    150
    • Abstract Views
      130
    • Downloads
      20

Article Description

Prevalent in marine, estuarine, and coastal environments, Vibrio parahaemolyticus is one of the major foodborne pathogens which can cause acute gastroenteritis through consumption of contaminated food. This study encompassed antimicrobial resistance, molecular characteristics, and phylogenetic relationships of 163 V. parahaemolyticus isolated from aquatic foods across 15 provinces in China. The isolates showed high resistance rates against ampicillin (90.80%, 148/163) and cefazolin (72.39%, 118/163). Only 5 isolates demonstrated multi-drug resistance (MDR) phenotypes. A total of 37 different antibiotic resistance genes (ARGs) in correlation with seven antimicrobial categories were identified in 163 isolates. tet(34) and tet(35) were present in all 163 isolates. Other most prevalent ARGs were those conferring resistance to β-lactams, with prevalence rate around 18.40% (30/163). The virulence genes tdh and trh were found in 17 (10.43%) and 9 (5.52%) isolates, respectively. Totally 121 sequence types (STs) were identified through whole genome analysis, among which 60 were novel. The most prevalent sequence type was ST3 (9.20%, 15/163), which shared the same genotype profile of trh-, tdh+ and blaCARB-22+. Most of the trh+ and/or tdh+ V. parahaemolyticus isolates were clustered into a distinctive clade by the phylogenetic analysis. Our study showed that the antimicrobial resistance of V. parahaemolyticus in aquatic foods in China was moderate. However, the emerging of MDR isolates implicate strengthened monitoring is needed for the better treatment of human V. parahaemolyticus infections. High genetic diversity and virulence potential of the isolates analyzed in this study help better understanding and evaluating the risk of V. parahaemolyticus posed to public health.

Bibliographic Details

Yao Bai; Fengqin Li; Shuran Yang; Dajin Yang; Zixin Peng; Jin Xu; Yinping Dong; Shaofei Yan; Ning Li; Qiuping Yang; Jiali Sun; Baowei Yang; Yanan Sun

Elsevier BV

Multidisciplinary; Vibrio parahaemolyticus; aquatic foods; antimicrobial susceptibility; genomic analysis

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