Naphthopyran-Based Photoswitching for Chemical Sensing and Imaging in Phase-Sensitive Mode
SSRN, ISSN: 1556-5068
2024
- 92Usage
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Intensity-based fluorescence imaging and sensing commonly suffer from spectral overlap and optical background interference. While fluorescence lifetime measurements in frequency domain offer an alternative, acquisition on the nanosecond level is largely constrained. Herein, we propose photoswitchable probes containing naphthopyran and fluorescent donors for interrogation in phase-sensitive detection mode. The method features reaction kinetics in the millisecond to second regime, which allows frequency domain detection with cost-effective equipment and offers an alternative to conventional fluorescence lifetime imaging. A phase shift (Δf) in the fluorescence of the probes was extracted by Fast Fourier Transform (FFT), establishing a dependence on the molar ratio of donor to acceptor. Thus, Δf is proposed as a self-referencing quantity for selective highlighting of fluorescent probes and as a dynamic signal readout in chemical sensing. Phase-sensitive detection of protamine, a polycationic protein often used as neutralizer of the anticoagulant heparin during surgery, was successfully realized based on the platform.
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