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Naphthopyran-Based Photoswitching for Chemical Sensing and Imaging in Phase-Sensitive Mode

SSRN, ISSN: 1556-5068
2024
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  • Usage
    92

Article Description

Intensity-based fluorescence imaging and sensing commonly suffer from spectral overlap and optical background interference. While fluorescence lifetime measurements in frequency domain offer an alternative, acquisition on the nanosecond level is largely constrained. Herein, we propose photoswitchable probes containing naphthopyran and fluorescent donors for interrogation in phase-sensitive detection mode. The method features reaction kinetics in the millisecond to second regime, which allows frequency domain detection with cost-effective equipment and offers an alternative to conventional fluorescence lifetime imaging. A phase shift (Δf) in the fluorescence of the probes was extracted by Fast Fourier Transform (FFT), establishing a dependence on the molar ratio of donor to acceptor. Thus, Δf is proposed as a self-referencing quantity for selective highlighting of fluorescent probes and as a dynamic signal readout in chemical sensing. Phase-sensitive detection of protamine, a polycationic protein often used as neutralizer of the anticoagulant heparin during surgery, was successfully realized based on the platform.

Bibliographic Details

Yu Cheng; Yifu Wang; Xiaojiang Xie; Jingying Zhai

Elsevier BV

Multidisciplinary; Photoswitch; Fluorescent Probe; Naphthopyran; Phase-sensitive; Protamine

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