PlumX Metrics
Embed PlumX Metrics

Geographical Variation in Metabolite Profiles and Bioactivity of Thesium Chinense Turcz. Revealed by UHPLC-Q/TOF-MS-Based Metabolomics

SSRN, ISSN: 1556-5068
2024
  • 0
    Citations
  • 81
    Usage
  • 0
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Usage
    81

Article Description

Thesium chinense Turcz. (TCT), a member of the Apiaceae family with a long-standing role in traditional Chinese medicine, is celebrated for its wide range of pharmacological properties such as antioxidant, antimicrobial, and anti-inflammatory effects. This study employs a non-targeted metabolomics approach via ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) to investigate the metabolite differences and quality characteristics in wild TCT specimens collected from seven provinces in China. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied to identify and differentiate the metabolite profiles of TCT samples, revealing 54 differential metabolites, predominantly flavonoids and alkaloids. The study further employed KEGG pathway analysis, indicating the greatest variations in the biosynthesis pathways of flavonoids and flavanols among the samples. Correlating the metabolite content with the geographical origins, a strong association was observed, suggesting that the bioactivity of TCT is significantly influenced by its provenance. The study also validated the antioxidant and anti-inflammatory activities of TCT, which were found to be highly predictive of the total flavonoid content (TFC). The findings of this research not only provide a comprehensive quality analysis and control framework for herbal medicine but also offer critical theoretical support for the evaluation of TCT quality from different regions. This work highlights the potential of metabolomics in discerning the subtleties of plant metabolomes, thereby contributing to the advancement of traditional Chinese medicine and its integration into modern healthcare practices.

Bibliographic Details

Niannian Zhou; Hao Yu; Hao Chen; Cong Wang; Guanglei Zhang; Fang Zhang; Ke Che; Tingting Sun; Haonan Xu; Mengyuan Chen; Qisheng Yao

Elsevier BV

Multidisciplinary; Thesium chinense Turcz.; UPLC-Q-TOF-MS/MS; geographical variations; non-targeted metabolomics; total flavonoid content; antioxidant; anti-inflammatory

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know