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Comparative Mrna Transcriptome Analysis of Mulberry Silkworm (Bombyx Mori L.) During Bmdv, Bmifv and Bmnpv Infection

SSRN, ISSN: 1556-5068
2024
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Article Description

Bombyx mori Densonucleosis (BmDV), Infectious flacherie (BmIFV) and nuclear polyhedrosis (BmNPV) virus infections in silkworms causes major crop losses in India. Defense related genes against these three viruses still remain unclear at the transcriptomic level in silkworms. PAM117 (virus-tolerant bivoltine silkworm strain) was reared and inoculated individually with BmDV, BmIFV and BmNPV immediately after second moult. The midgut samples of 3rd day Vth instar silkworm larvae were dissected and RNA was extracted. Transcriptome sequencing was performed on Illumina NovaSeq 6000 sequencer. Differential expression gene analysis indicated that 3624 genes were up-regulated and 3187 genes down-regulated for BmDV, whereas 1488 genes were up-regulated and 948 genes down-regulated for BmIFV and 2320 genes were up-regulated and 526 genes down-regulated for BmNPV inoculated samples. BGIBMGA004285, BGIBMGA004399, BGIBMGA013803 and BGIBMGA002739 genes had strong positive correlation with the qPCR and transcriptome data. These results further add to the existing information on the understanding of enhanced virus tolerance in silkworms.

Bibliographic Details

Satish Lokanath; Kusuma Lingaiah; Moorthy Shanmugam Manthira; Sivaprasad Vankadara

Elsevier BV

Multidisciplinary; Bombyx mori; Midgut; Virus; Transcriptome; Tolerant; Silkworm

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