In vitro exposure of Nile tilapia (Oreochromis niloticus) testis to estrogenic endocrine disrupting chemicals: mRNA expression of genes encoding steroidogenic enzymes
Toxicology Mechanisms and Methods, ISSN: 1537-6516, Vol: 22, Issue: 1, Page: 47-53
2012
- 15Citations
- 36Captures
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Metrics Details
- Citations15
- Citation Indexes14
- 14
- CrossRef8
- Policy Citations1
- Policy Citation1
- Captures36
- Readers36
- 36
Article Description
Many environmental pollutants can exert adverse effects on exposed organisms, including fish, leading to disruption of the endocrine system. Enzymes involved in the sex steroid biosynthesis are potential targets for the toxic action of pollutants. In this context, we investigated the hypothesis that selected estrogenic chemicalsthe pharmaceutical estrogen ethinylestradiol (EE2), the phytoestrogen genistein (GEN), and the industrial compound bisphenol A (BPA)may cause endocrine disruption by directly disturbing steps of fish steroidogenic pathways. We studied the mRNA expression of eight selected genes encoding steroidogenic enzymes (11β-HSD2, 20β-HSD, 3β-HSD1, 17β-HSD1, 17β-HSD8, 17β-HSD12, CYP19a, CYP19b) by quantitative real-time PCR. Testis slices from adult specimens of the model fish Nile tilapia were exposed in vitro for 3 and 8h either to individual or to mixture solutions of EE2 (100ng/L), GEN (200ng/L), and BPA (10 g/L); all at the peak concentrations observed in the Douro River estuary (Portugal). Our data revealed that only the mixture of the tested chemicals directly induced the expression of 11β-HSD2, 17β-HSD1, and 17β-HSD12, after 8h, whereas no effect was seen for chemicals tested individually. The gene expression pattern agrees with the concept of dose addition for environmental mixtures, and for the first time an interference of estrogenic EDCs is reported for 17β-HSD1 and 17β-HSD12. © 2012 Informa Healthcare USA, Inc.
Bibliographic Details
Informa UK Limited
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