Extraction and Identification of Phenolic Compounds from the Iraqi Heliotropium europaeum L. plant
Iraqi Journal of Pharmaceutical Sciences, ISSN: 2521-3512, Vol: 30, Issue: 2, Page: 158-166
2021
- 3Citations
- 7Captures
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Article Description
The Heliotropium europaeum L. (Boraginaceae) are well-known to contain toxic pyrrolizidine alkaloids in addition to other secondary metabolites. Its spread in the Mediterranean area, southern Europe, Central Asia, and Iraq. The present study included extraction of the aerial parts of Iraqi Heliotropium europaeum with methanol (soxhlet apparatus), fractionation, screening the active constituent, and identification by chromatographic techniques. The extract was suspended in distilled water and partitioned with chloroform, ethyl acetate, and n-butanol. The hydrolysis step was done for the two fractions (n-butanol and ethyl acetate). Phytochemical screening and identification of bioactive substances of the plant was done for the two fractions. A qualitative and quantitative analysis of the fractions was carried using a high -performance liquid chromatography technique and twenty–one reference standards. The outcomes of this study were the identifications of new six phenolic compounds (kaempferol (1), Silybin (2), caffeic acid (3), Genistein (4), Apigenin (5), in addition to syringic acid (6)) from Heliotropium europaeum ethyl acetate fraction.
Bibliographic Details
College of Pharmacy University of Baghdad
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