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Quantitative Differences in Ia Antigen Expression in the Spleens of 15I 5 - B Congenic and Inbred Chickens as Defined by a New Monoclonal Antibody

Poultry Science, ISSN: 0032-5791, Vol: 67, Issue: 11, Page: 1525-1535
1988
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  • Citations
    17
  • Captures
    3
  • Social Media
    178
    • Shares, Likes & Comments
      178
      • Facebook
        178

Article Description

A monoclonal antibody (MAb), designated P2M11, that detects a monomorphic determinant of chicken class II antigens was produced from the fusion of P3X63 myeloma cells with spleen cells from BALB/c mice immunized with chicken splenic lymphocytes. Flow cytometric analyses of lymphocytes from the SC and FP strains of chickens showed 30 to 50% staining of bursa cells, 15 to 20% staining cells, and less than 5% staining of thymus cells. Addition of MAb P2M11 to splenic T cell cultures stimulated with allogeneic cells or concanavalin A resulted in a significant inhibition of the T cell proliferation responses. Immunoprecipitation of 35 S-methionine-labeled spleen cell extracts using MAb P2M11 identified molecules with apparent molecular weights of approximately 28,000, 30,000, and 32,000 by sodium dodecyl-polyacryl-amide gel electrophoresis. Taken together, these data indicate that the antigens detected by MAb P2M11 are similar in cell distribution and structure to chicken Ia antigens encoded by B-L genes. Using this MAb, a strain difference was demonstrated in the tissue distribution of Ia antigen positive lymphocytes in the spleens but not the thymuses of 15I 5 - B congenic and inbred strains of chickens. This difference may be due to the genes associated with B -complex genes.

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