Development of KASP Markers and Identification of a QTL Underlying Powdery Mildew Resistance in Melon (Cucumis melo L.) by Bulked Segregant Analysis and RNA-Seq
Frontiers in Plant Science, ISSN: 1664-462X, Vol: 11, Page: 593207
2021
- 38Citations
- 39Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations38
- Citation Indexes38
- 38
- CrossRef13
- Captures39
- Readers39
- 39
Article Description
Powdery mildew (PM), caused by Podosphaera xanthii (Px), is one of the most devastating fungal diseases of melon worldwide. The use of resistant cultivars is considered to be the best and most effective approach to control this disease. In this study, an F segregating population derived from a cross between a resistant (wm-6) and a susceptible cultivar (12D-1) of melon was used to map major powdery mildew resistance genes using bulked segregant analysis (BSA), in combination with next-generation sequencing (NGS). A novel quantitative trait locus (QTL) named qCmPMR-12 for resistance to PM on chromosome 12 was identified, which ranged from 22.0 Mb to 22.9 Mb. RNA-Seq analysis indicated that the MELO3C002434 gene encoding an ankyrin repeat-containing protein was considered to be the most likely candidate gene that was associated with resistance to PM. Moreover, 15 polymorphic SNPs around the target area were successfully converted to Kompetitive Allele-Specific PCR (KASP) markers (P < 0.0001). The novel QTL and candidate gene identified from this study provide insights into the genetic mechanism of PM resistance in melon, and the tightly linked KASP markers developed in this research can be used for marker-assisted selection (MAS) to improve powdery mildew resistance in melon breeding programs.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85101242181&origin=inward; http://dx.doi.org/10.3389/fpls.2020.593207; http://www.ncbi.nlm.nih.gov/pubmed/33613580; https://www.frontiersin.org/articles/10.3389/fpls.2020.593207/full; https://www.frontiersin.org/articles/10.3389/fpls.2020.593207/supplementary-material/10.3389/fpls.2020.593207.s001; http://dx.doi.org/10.3389/fpls.2020.593207.s001; https://dx.doi.org/10.3389/fpls.2020.593207.s001; https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.593207/full; https://dx.doi.org/10.3389/fpls.2020.593207
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