Daytime, Not Nighttime, Elevated Atmospheric Carbon Dioxide Exposure Improves Plant Growth and Leaf Quality of Mulberry (Morus alba L.) Seedlings

Almost all elevated atmospheric CO concentrations (eCO) studies have not addressed the potential responses of plant growth to different CO in daytime and nighttime. The present study was to determine the impact of daytime and/or nighttime eCO on growth and quality of mulberry (Morus alba L.), a perennial multipurpose cash plant. Six-month-old mulberry seedlings were hence grown in environmentally auto-controlled growth chambers under four CO concentrations: (1) ambient CO (ACO, 410 μmol mol daytime/460 μmol mol nighttime), (2) sole daytime elevated CO (DeCO, 710 μmol mol/460 μmol mol), (3) sole nighttime elevated CO (NeCO, 410 μmol mol/760 μmol mol), and (4) continuous daytime and nighttime elevated CO (D + NeCO, 710 μmol mol/760 μmol mol). Plant growth characteristics, nutrient uptake, and leaf quality were then examined after 120 days of CO exposure. Compared to control, DeCO and (D + N)eCO increased plant biomass production and thus the harvest of nutrients and accumulation of leaf carbohydrates (starch, soluble sugar, and fatty acid) and N-containing compounds (free amino acid and protein), though there were some decreases in the concentration of leaf N, P, Mg, Fe, and Zn. NeCO had no significant effects on leaf yield but an extent positive effect on leaf nutritional quality due to their concentration increase in leaf B, Cu, starch, and soluble sugar. Meanwhile, (D + N)eCO decreased mulberry leaf yield and harvest of nutritious compounds for silkworm when compared with DeCO. The reason may be associated to N, P, Mg, Fe, and Zn that are closely related to leaf pigment and N metabolism. Therefore, the rational application of mineral nutrient (especially N, P, Fe, Mg, and Zn) fertilizers is important for a sustainable mulberry production under future atmosphere CO concentrations.