Laetiporus sulphureus Mushroom Enhances Cytotoxic Effect of Bifidobacterium animalis spp. lactis on HCT-116 Cells in a Co-Culture System †
Engineering Proceedings, ISSN: 2673-4591, Vol: 56, Issue: 1
2024
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Article Description
The study aimed to test the effect of probiotic Bifidobacterium animalis spp. lactis (BAL) on the HCT-116 cell line viability and to compare its effect with co-treatment BAL/Laetiporus sulphureus (EALS). The trypan blue staining method was used to estimate HCT-116 viability. The levels of NO were determined using 0.1% N-(1-naphthyl) ethylenediamine, as well as 1% sulfanilic acid. The determination of HO was based on the oxidation of phenol red. Our results showed the significant cytotoxicity of the BAL on the HCT-116 cells in a co-culture system, while the BAL/EALS co-treatment further enhanced the cytotoxicity on the HCT-116 cells. We detected higher HO and NO values in treatments with BAL, especially in the BAL/EALS co-treatment. The death of the HCT-116 cells may be due to elevated levels of HO and NO and their products (peroxynitrites).
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