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Amperometric Miniaturised Portable Enzymatic Nanobiosensor for the Ultrasensitive Analysis of a Prostate Cancer Biomarker

Journal of Functional Biomaterials, ISSN: 2079-4983, Vol: 14, Issue: 3
2023
  • 8
    Citations
  • 0
    Usage
  • 19
    Captures
  • 2
    Mentions
  • 47
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    8
    • Citation Indexes
      8
  • Captures
    19
  • Mentions
    2
    • Blog Mentions
      1
      • Blog
        1
    • News Mentions
      1
      • News
        1
  • Social Media
    47
    • Shares, Likes & Comments
      47
      • Facebook
        47

Most Recent News

Slovak Academy of Sciences Researcher Furthers Understanding of Biosensors (Amperometric Miniaturised Portable Enzymatic Nanobiosensor for the Ultrasensitive Analysis of a Prostate Cancer Biomarker)

2023 APR 04 (NewsRx) -- By a News Reporter-Staff News Editor at Biotech News Daily -- Investigators publish new report on biosensors. According to news

Article Description

Screen-printing technology is a game changer in many fields including electrochemical biosensing. Two-dimensional nanomaterial MXene TiCT was integrated as a nanoplatform to immobilise enzyme sarcosine oxidase (SOx) onto the interface of screen-printed carbon electrodes (SPCEs). A miniaturised, portable, and cost-effective nanobiosensor was constructed using chitosan as a biocompatible glue for the ultrasensitive detection of prostate cancer biomarker sarcosine. The fabricated device was characterised with energy-dispersive X-ray spectroscopy (EDX), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Sarcosine was detected indirectly via the amperometric detection of HO formed during enzymatic reaction. The nanobiosensor could detect sarcosine down to 7.0 nM with a maximal peak current output at 4.10 ± 0.35 × 10 A using only 100 µL of a sample per measurement. The assay run in 100 μL of an electrolyte showed the first linear calibration curve in a concentration window of up to 5 μM with a slope of 2.86 μA·μM, and the second linear calibration curve in the range of 5–50 μM with a slope of 0.32 ± 0.01 μA·μM (R = 0.992). The device provided a high recovery index of 92.5% when measuring an analyte spiked into artificial urine, and could be used for detection of sarcosine in urine for at least a period of 5 weeks after the preparation.

Bibliographic Details

Hroncekova, Stefania; Lorencova, Lenka; Bertok, Tomas; Hires, Michal; Jane, Eduard; Bučko, Marek; Kasak, Peter; Tkac, Jan

MDPI AG

Materials Science; Engineering

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