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Phosphorylation of Calpastatin Negatively Regulates the Activity of Calpain

Life, ISSN: 2075-1729, Vol: 13, Issue: 3
2023
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Research from Institute of Food Science and Technology in Life Sciences Provides New Insights (Phosphorylation of Calpastatin Negatively Regulates the Activity of Calpain)

2023 APR 07 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- Research findings on life sciences are discussed in

Article Description

Tenderness is an important characteristic of meat quality. Calpastatin and calpain play important roles in meat tenderization. However, it is not clear how phosphorylation affects the regulation of calpastatin on μ-calpain and, consequently, meat tenderness. Calpastatin with high and low phosphorylation levels were obtained in vitro corresponding to the treatments by protein kinase A (PKA) and alkaline phosphatase. Then, calpain was incubated with calpastatin with different phosphorylation levels, and the effect of calpastatin on calpain activity under different phosphorylation levels was analyzed. The results showed that PKA promoted the phosphorylation of calpastatin, and a high phosphorylation level was maintained during incubation. The degradation rate of μ-calpain in AP group was higher than that in the other groups, meaning there was lower inhibition of calpastatin on calpain activity. The degradation of calpastatin was lower and its structure was more stable after phosphorylation. One more serine 133 site of calpastatin was identified in PKA group compared with the other groups. Phosphorylation at serine 133 of calpastatin enhanced its inhibition on calpain activity by maintaining its structural stability, thus inhibiting the tenderization of meat.

Bibliographic Details

Bai, Yuqiang; Hou, Chengli; Huang, Caiyan; Fang, Fei; Dong, Yu; Li, Xin; Zhang, Dequan

MDPI AG

Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Earth and Planetary Sciences

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