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Effects of manganese porphyrins on cellular sulfur metabolism

Molecules, ISSN: 1420-3049, Vol: 25, Issue: 4
2020
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Article Description

Manganese porphyrins (MnPs),MnTE-2-PyP, MnTnHex-2-PyP and MnTnBuOE-2-PyP, are superoxide dismutase (SOD) mimetics and forma redox cycle between O and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (HO). We previously found that MnPs oxidize hydrogen sulfide (HS) to polysulfides (PS; HSn, n = 2-6) in buffer. Here, we examine the effects of MnPs for 24 h on HS metabolismand PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using HS (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular HS production and increased intracellular PS. HS metabolism and PS production were unaffected by cellular O (5% versus 21% O), HO or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of HS production in HEK293 cells and that MnPs decrease mitochondrial HS production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of HS to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.

Bibliographic Details

Olson, Kenneth R; Gao, Yan; Steiger, Andrea K; Pluth, Michael D; Tessier, Charles R; Markel, Troy A; Boone, David; Stahelin, Robert V; Batinic-Haberle, Ines; Straubg, Karl D

MDPI AG

Chemistry; Biochemistry, Genetics and Molecular Biology; Pharmacology, Toxicology and Pharmaceutics

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