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A novel non-digestible, carrot-derived polysaccharide (Crg-i) selectively modulates the human gut microbiota while promoting gut barrier integrity: An integrated in vitro approach

Nutrients, ISSN: 2072-6643, Vol: 12, Issue: 7, Page: 1-22
2020
  • 53
    Citations
  • 0
    Usage
  • 53
    Captures
  • 0
    Mentions
  • 23
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    53
  • Captures
    53
  • Social Media
    23
    • Shares, Likes & Comments
      23
      • Facebook
        23

Article Description

Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in vitro models. First, digestion methods involving α-amylase/brush border enzymes demonstrated the non-digestibility of cRG-I by host-derived enzymes versus digestible (starch/maltose) and non-digestible controls (inulin). Then, a recently developed short-term (48 h) colonic incubation strategy was applied and revealed that cRG-I fermentation increased levels of health-promoting short-chain fatty acids (SCFA; mainly acetate and propionate) and lactate comparable but not identical to the reference prebiotic inulin. Upon upgrading this fermentation model by inclusion of a simulated mucosal environment while applying quantitative 16S-targeted Illumina sequencing, cRG-I was additionally shown to specifically stimulate operational taxonomic units (OTUs) related to health-associated species such as Bifidobacterium longum, Bifidobacterium adolescentis, Bacteroides dorei, Bacteroides ovatus, Roseburia hominis, Faecalibacterium prausnitzii, and Eubacterium hallii. Finally, in a novel model to assess host–microbe interactions (Caco-2/peripheral blood mononuclear cells (PBMC) co-culture) fermented cRG-I increased barrier integrity while decreasing markers for inflammation. In conclusion, by using novel in vitro models, cRG-I was identified as a promising prebiotic candidate to proceed to clinical studies.

Bibliographic Details

Van den Abbeele, Pieter; Verstrepen, Lynn; Ghyselinck, Jonas; Albers, Ruud; Marzorati, Massimo; Mercenier, Annick

MDPI AG

Agricultural and Biological Sciences; Nursing

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