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Intra-Palpebral Tuberculin Skin Test and Interferon Gamma Release Assay in Diagnosing Tuberculosis due to Mycobacterium caprae in European Bison (Bison bonasus)

Pathogens, ISSN: 2076-0817, Vol: 11, Issue: 2
2022
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Article Description

Despite the threat posed by tuberculosis (TB) to the protected European bison (Bison bona-sus), no validated TB tests exist for this species. This pilot study evaluates two tests based on detecting cellular immunity for this purpose: interferon gamma release assay (IGRA) and tuberculin skin test (TST). Ten animals were subjected to ante-mortem and post-mortem examinations. IGRA was performed using a commercial test, and the comparative TST was performed in the eyelids. The lesions were assessed post-mortem and material was collected for mycobacterial culture. The isolated strains were subjected to genotyping. At post-mortem examination, five out of ten individuals demonstrated both tuberculous lesions and positive culture results (Mycobacterium caprae). Com-pared to the palpebral TST, the findings of the IGRA are easier to interpret when diagnosing tuberculosis in European bison.

Bibliographic Details

Didkowska, Anna; Orłowska, Blanka; Krajewska-Wędzina, Monika; Krzysiak, Michał; Bruczyńska, Małgorzata; Wiśniewski, Jan; Klich, Daniel; Olech, Wanda; Anusz, Krzysztof

MDPI AG

Medicine; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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