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Time-resolved fluorescent immunochromatography of aflatoxin B1 in soybean sauce: A rapid and sensitive quantitative analysis

Sensors (Switzerland), ISSN: 1424-8220, Vol: 16, Issue: 7, Page: 1094
2016
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Article Description

Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg•kg, with a limit of detection (LOD) of 0.1 µg•kg and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg•kg. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

Bibliographic Details

Wang, Du; Zhang, Zhaowei; Li, Peiwu; Zhang, Qi; Zhang, Wen

MDPI AG

Chemistry; Biochemistry, Genetics and Molecular Biology; Physics and Astronomy; Engineering

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